taq dna polymerase protocol

Takara ex taq dna polymerase combines the proven performance of takara taq polymerase with the proofreading activity of an efficient 3 to 5 exonuclease for high sensitivity high efficiency pcr reactions it can also be used for long range pcr up to 20 kb from genomic dna templates and up to 30 kb from lambda dna templates. Platinum taq dna polymerase high fidelity is ideal for amplification of dna fragments when high yields and robust amplification are required. Its name is often abbreviated to taq or taq pol it is frequently used in the polymerase chain reaction pcr a method for greatly amplifying the quantity of short segments of dna.
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High fidelity is provided by a mixture of platinum taq dna polymerase and the proofreading 3 5 exonuclease activity enzyme pyrococcus species gb d polymerase. See also table 1. Protocols io also provides an interactive version of this protocol where you can discover and share optimizations with the research community.

In house we have tested fragments up to 13 kb from complex genomic dna or up to 30 kb from less complex lambda phage dna using this protocol.

Taq dna polymerase protocol. Ex taq polymerase has a higher fidelity than standard taq with a. However nowadays high fidelity taq dna polymerase specific taq dna polymerase and high sensitive dna polymerases are commercially available depending upon the type of pcr reaction. Rr002a takara la taq dna polymerase mg2 free buffer 125 units. Product size price license quantity details.

Pcr protocol for taq dna polymerase with standard taq buffer m0273. Taq dna polymerase is a thermostable dna polymerase that possesses a 5 3 polymerase activity 1 2 3 and a 5 flap endonuclease activity 4 5. Protocols io also provides an interactive version of this protocol where you can discover and share optimizations with the research community. The taq dna polymerase always needs an mg 2 ion as a cofactor.

Takara la taq is a mixture of taq polymerase with a proofreading polymerase. Optimal polymerization activity is achieved at 75 80 c with half maximal activity at 60 70 c lawyer et al 1993. Pcr the polymerase chain reaction pcr is a powerful and sensitive technique for dna amplification 1. The mixture is optimized for amplification of long dna templates routinely 20 kb and up to 48 kb depending on template type with high fidelity.

Supplied with 10x reaction buffer. It is supplied with 10x standard taq reaction buffer which is detergent free and designed to be compatible with existing assay systems. Choosing the right dna polymerase for your pcr experiment. Read our article on it.

Pcr the polymerase chain reaction pcr is a powerful and sensitive technique for dna amplification 1. Pcr specificity is improved with the incorporation of platinum automatic hot. Pcr protocol for taq dna polymerase with standard taq buffer m0273. Taq polymerase is a thermostable dna polymerase i named after the thermophilic eubacterial microorganism thermus aquaticus from which it was originally isolated by chien et al.

Isolated from a recombinant source. The protocol uses a mixture of hotstar hifidelity dna polymerase and taq or hotstar taq plus dna polymerase and allows much longer fragments to be generated. Taq dna polymerase is an 832 amino acid protein with an inferred molecular weight of 93 920 and a specific activity of 292 000 units mg.