taq dna polymerase exonuclease activity

Isolated from a recombinant source.

Taq dna polymerase exonuclease activity. Yes extra a. 10 min at 97 c. It is a single polypeptide chain with a molecular weight of approximately 95 kda. Pcr specificity is improved with the incorporation of platinum automatic hot.

Optimal polymerization activity is achieved at 75 80 c with half maximal activity at 60 70 c lawyer et al 1993. During this process dna polymerase reads the existing dna strands to create two new. This mixture of enzymes allows for long and accurate la pcr of targets from a variety of templates including genomic dna. Takara ex taq dna polymerase combines the proven performance of takara taq polymerase with the proofreading activity of an efficient 3 to 5 exonuclease for high sensitivity high efficiency pcr reactions it can also be used for long range pcr up to 20 kb from genomic dna templates and up to 30 kb from lambda dna templates.

Taq dna polymerase is a highly processive 5 3 dna polymerase that lacks 3 5 exonuclease activity. A dna polymerase is a member of a family of enzymes that catalyze the synthesis of dna molecules from nucleoside triphosphates the molecular precursors of dna these enzymes are essential for dna replication and usually work in groups to create two identical dna duplexes from a single original dna duplex. 60 min at 94 c amplification efficiency. It is supplied with 10x standard taq reaction buffer which is detergent free and designed to be compatible with existing assay systems.

2 4 kb min at 72 c half life. Takara la taq dna polymerase combines taq dna polymerase and a dna proofreading polymerase with 3 5 exonuclease activity to enable pcr amplification of very long dna templates long range pcr. Taq dna polymerase is an 832 amino acid protein with an inferred molecular weight of 93 920 and a specific activity of 292 000 units mg. The dna polymerase has two important activity during replication.

10 5 fold 5 3 exonuclease activity. Its name is often abbreviated to taq or taq pol it is frequently used in the polymerase chain reaction pcr a method for greatly amplifying the quantity of short segments of dna. Platinum taq dna polymerase high fidelity is ideal for amplification of dna fragments when high yields and robust amplification are required. Supplied with 10x reaction buffer.

High fidelity is provided by a mixture of platinum taq dna polymerase and the proofreading 3 5 exonuclease activity enzyme pyrococcus species gb d polymerase. Dntp ddntp dutp biotin 11 dutp dig 11 dutp fluorescent dntp ddntp extension rate. 5 units µl recombinant enzyme. La taq dna polymerase is supplied with optimized la pcr buffer ii.

Taq dna polymerase was originally isolated from the thermophilic eubacterium thermus aquaticus bm a strain lacking taq i. Taq polymerase is a thermostable dna polymerase i named after the thermophilic eubacterial microorganism thermus aquaticus from which it was originally isolated by chien et al.