t4 dna ligase takara

10 t4 dna ligase buffer 1 ml.

T4 dna ligase takara. Takara s dna ligation kit mighty mix is a one solution premix ligation reagent that offers high efficiency ligations particularly for blunt ended ligation. Ligation protocol with t4 dna ligase. T4 dna ligase catalyzes the formation of phosphodiester bonds between double stranded dna fragments with 3 oh and 5 phosphate ends in the presence of atp. Clontech takara q a.

Dna ligation kit mighty mix 高効率 1液タイプのライゲーションキット. The dna ligase seals nicks in the assembled dna. 10x t4 dna ligase buffer.

Dna fragments encoding g3bp1 or g3bp2 were inserted into the pegfp c3 backbone using nebuilder hifi dna assembly master mix kit neb e2621. The reaction can be performed for 5 minutes at 25 c rapid protocol or 30 minutes at 16 c standard protocol with equal results for both circular and linear dna ligations. The end result is a double stranded fully sealed dna molecule that can serve as template for pcr rca or a variety of other molecular biology applications including direct transformation of e coli.

T4 dna ligase 350 u μl 25 000 u. Nebuilder hifi dna assembly cloning kit is supplied with neb 5 alpha high efficiency competent e. Escherichia coli carrying the plasmid that enables highly expression of t4 dna ligase gene. The fkbp f36m fragment was pcr amplified from plvx rhom 1 takara 635062.

The cdna library was created by superscript iii first strand synthesis system thermo 18080051.