T4 dna ligaseそのものの酵素反応至適温度は37 c であり 反応温度を37 c に近づけることはライゲーション反応の効率が上がることを期待できますが 同時に非特異的ライゲーション等が起こる可能性も上昇し ライゲーション反応の正確性は低下する危険性. T4 gt7 dna t4dc dna 318 03971.
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