quick load 100 bp dna ladder

These ladders have reference bands at 1000 bp and 3000 bp for easy orientation.

Quick load 100 bp dna ladder. 1 kb ladder can be bought commercially at various concentrations but the recommended load for an electrophoresis run is 0 5 µg 5µl. Specifications analytical specifications d5000 screentape assay and reagents sizing range 100 5000 bp typical resolution 400 5000 bp. Choose the sonication conditions that generate the optimal dna fragment size of 100 600 bp and use for preparation of the input sample in section iv step 4. Invitrogen trackit 100 bp dna ladder is ready to use and designed for sizing and approximate quantification of double stranded dna in the range of 100 bp to 2 000 bp.

250 800 ng per microliter sounds like a lot of dna that should be visible on a gel. The following reagents are supplied with this product. This product is available in quick load purple format enjoy the convenience of a ready to load format. 100 bp dna ladder consists of 13 individual chromatography purified dna fragments and has reference bands at 2000 1500 and 600 bp for easy orientation.

Neb also offers a quick load version of this ladder with purple dye. The d5000 screentape system is designed for analysing dna molecules from 100 5000 bp. A single convenient ladder for 100 bp up to 10 kb 100 bp and 1 kb ladder in one value pricing. 100 bp dna ladder visualized by ethidium bromide staining on a 1 3 tae agarose gel.

Quick load purple 100 bp dna ladder is stable for at least 6 months at 25 c. Neb also offers a quick load version of this ladder with purple dye. A dye free loading buffer 30 glycerol is recommended to better observe the dna smear on gel. The quick load purple 100 bp dna ladder is not intended for precise quantification of dna mass but can be used for approximating the mass of dna in comparably intense samples of similar size.

Recommended gel percentage range. Optimum separation on 2. Invitrogen 100 bp dna ladder is designed for sizing and approximate quantification of double stranded dna in the range of 100 bp to 2 000 bp. 100 bp dna ladder visualized by ethidium bromide staining on a 1 3 tae agarose gel.

The following reagents are supplied with this product. Mass values are for 0 5 µg lane. Optimum separation on 2. However you would expect a smear on the gel since genomic dna contains fragments of different length.