qubit dna hs assay kit

The qubit dsdna hs high sensitivity assay kit is designed specifically for use with the qubit fluorometer.

Qubit dna hs assay kit. Hi i m using qubit to quantify rna and also dna for ngs. If the concentration reads too low then use the quant it dsdna hs kit and repeat section 2 and use the and keys to highlight quant it dna hs assay. Dsdna hs high sensitivity assay kits make dna quantitation easy and accurate. Size of dna in bp.

Up to 11 samples chip. The qubit fluorometer uses fluorescent dyes to determine the concentration of either nucleic acids or proteins in a sample. The assay is highly selective for double stranded dna dsdna over rna and is designed to be accurate for initial sample concentrations from 10 pg µl to 100 ng µl. Up to 11 samples chip.

Simply dilute the reagent using the buffer provided add your sample any volume from 1 20 μl is acceptable then read the concentration using the qubit. The assay is highly selective for rna over double stranded dna dsdna figure 1 page 7 and is accurate for initial sample concentrations from 250 pg µl to 100 ng µl. Browse our listings to find jobs in germany for expats including jobs for english speakers or those in your native language. The other common method of measuring the concentration of nucleic acids and protein is the uv absorbance method which uses a spectrophotometer to measure the natural absorbance of light at 260 nm for dna and rna or 280 nm for proteins.

I read in the guideline of hs rna measurement that to determine the purity of your sample i can use the qubit rna hs assay kit. Enter values and click button. 0 5 4 ng ul sensitivity 50 7000 bp size range. The assay is performed at room temperature and the signal.

If the concentration reads too high then dilute the sample to 200 500ng µl with te buffer ph 8 0 and repeat section 2 with quant it dna br assay. The qubit 1x dsdna hs high sensitivity assay kit is designed specifically for use with the qubit fluorometer.