Soils harbour some of the most diverse microbiomes on earth and are essential for both nutrient cycling and carbon storage. Research in underground sites has focused on mesophilic airborne fungi and only a few concerned cold adapted species. Dna was extracted from soil samples using the mp fastdna spin kit for soil mp biomedicals solon oh usa as per the manufacturer s instructions.
Most underground ecosystems are heterotrophic fungi in these objects are dispersed in the air in the form of spores and they may be potentially hazardous to mammals.

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