pfu dna polymerase

The enzyme replicates dna at 75 c catalyzing the polymerization of nucleotides in the 5 3 direction in the presence of magnesium. Pfu dna polymerase is an enzyme found in the hyperthermophilic archaeon pyrococcus furiosus where it functions to copy the organism s dna during cell division. Typically 0 5 2 5 units of enzyme are used per 50 μl reaction.
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Pfu dna polymerase is a thermostable enzyme that replicates dna at 75 c. Pfu dna polymerase a proofreading dna polymerase isolated from pyrococcus furiosus is an ideal choice for a variety of techniques requiring high fidelity dna synthesis by the polymerase chain reaction pcr. Dna polymerase also exhibits 3 5 exonuclease.

Abstract using compartmentalized self replication csr we evolved a version of pyrococcus furiosus pfu dna polymerase that tolerates modification of the γ phosphate of an incoming nucleotide.

Pfu dna polymerase. Our pfuturbo dna polymerase is a special formulation of cloned pfu dna polymerase and patented archaemaxx polymerase enhancing factor that has been shown to increase pcr product yields up to 19 kb without affecting replication fidelity. Other enzymes such as dna polymerase from pyrococcus furiosus pfu dna polymerase or thermoccocus litoralis vent dna polymerase new england biolabs have been reported to have a lower error rate than taq polymerase and may be advantageous for some applications. Phusion dna polymerase was developed by finnzymes oy now a part of thermo fisher scientific. Under agreement with and under the performance specifications of thermo fisher scientific.

Thermo scientific pfu dna polymerase is a highly thermostable dna polymerase from the hyperthermophilic archaeum pyrococcus furiosus. Pfu dna polymerase mastermix 2x is a premixed ready to use solution containing pfu dna polymerase dntps mgso 4 and reaction buffer at optimal concentrations for efficient amplification of dna templates by pcr. To prepare the final pcr only primers and template dna are added. It catalyzes the polymerization of nucleotides into duplex dna in the 5 3 direction in the presence of magnesium.

Pfu dna polymerase is a high fidelity thermostable enzyme of approximately 90kda isolated from pyrococcus furiosus. Pfu dna polymerase also possesses 3 5 exonuclease proofreading activity. The archaemaxx factor improves the yield of products by overcoming dutp poisoning which is caused by dutp accumulation during pcr through dctp deamination. This product is manufactured by new england biolabs inc.

In the laboratory setting pfu is used to amplify dna in the polymerase chain reaction pcr where the enzyme serves the central function of copying a new strand of dna during each extension step. The enzyme catalyzes the template dependent polymerization of nucleotides into duplex dna in the 5 3 direction.