pcold tf dna sequence

Coli vector with a multiple cloning site mcs for dna cloning. The insertion site is flanked by bstzi sites. The pet24a vector was purchased from invitrogen carlsbad ca usa.
dna micro aray application dna methylation hepatocyte dna methylation kit dna minimum spanning tree dna models leicester dna microarray technology dna microarray dna methylation guo 2014 landscape

Parental vector for ta cloning of pcr products. Pact and pbind were purchased from promega madison wi usa. Pcold tf dna was purchased from takara beijing china.

The mcs is reversed in puc18.

Pcold tf dna sequence. Then the daov gene was amplified by pcr and inserted into the pcold tf vector takara bio dalian china using the clonexpress ii one step cloning kit vazyme biotech nanjing china. Promoter for bacteriophage t7 rna polymerase. One to three copies of the dna target sequence are cloned into the pabai reporter vector which is then integrated into the y1hgold genome to create a bait specific reporter strain. Full length gif1 and slr1 cdnas were amplified and cloned into the pcold tf.

Screening for protein dna interactions with the matchmaker gold one hybrid system screening for protein dna interactions with the matchmaker gold one hybrid system. The puc19 plasmid was purchased from weidi biotechnology. This vector is also known as pgem 5zf. The map notes and annotations on this page and in the sequence map file are copyrighted material.

Nucleotide 1 and close tga nucleotide 3385 of grf4 are shown together with the protein coding dna sequence. Pcdna3 1 egfp pcdna3 1 mcherry pcmv rxrα and pcmv p85α were all gifts from xiaokun zhang.