oligo dna transfection

A high efficiency low toxicity transfection reagent optimized for oligonucleotide delivery into a wide range of cell types. Product number product description sds. On the day of transfection just prior to preparing complexes plate 1 0 3 5 x10 6 cells in 2 ml of growth medium without antibiotics per well.
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A n t n c n and g n are the number of each respective nucleotide within the polynucleotide. Popular taqman real time pcr assays antibodies oligos primers probes geneart gene synthesis cell culture plastics. The oligocalc dna mw calculations assume that there is not a 5 monophosphate anhydrous molecular weight a n x 313 21 t n x 304 2 c n x 289 18 g n x 329 21 61 96.

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Oligo dna transfection. Upload up to 200 oligo sequences at one time using the excel spreadsheet template below. For each transfection sample prepare dna. Page 1 of 1. The total volume of transfection complex medium dna and viafect transfection reagent to add per well of a 96 well plate is 5 10μl.

Enter names and sequences into the template save as an xls xlsx csv or txt file and upload when complete. The following protocol is a guideline for transfecting approximately 10 20 wells depending on the volume of viafect transfection reagent dna mixture used. Browse our new website to find cutting edge products to simplify and enhance your research immediately. Design and order custom oligos.

Dna molecular weight typically for synthesized dna oligonucleotides. For additional wells scale volumes accordingly. Restriction digestion can result in the production of blunt ends ends of a dna molecule that end with a base pair or sticky ends ends of a dna molecule that end with a nucleotide overhang. Mir 2164 mir 2160 mir 2165 mir 2166.

This website uses cookies to help provide you with the best possible online experience. Restriction digestion is usually used to prepare a dna fragment for subsequence molecular cloning as the procedure allows fragments of dna to be pieced. Showing 1 1 of 1 result for custom dna oligo advanced search. One day before transfection plate 0 25 1 x 10 6 cells in 2 ml of growth medium without antibiotics per well so that they will be 90 95 confluent at the time of transfection.

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