mg taq dna polymerase

Supplied with 10x reaction buffer.

Mg taq dna polymerase. It is a single polypeptide chain with a molecular weight of approximately 95 kda. Taq dna polymerase is a highly processive 5 3 dna polymerase that lacks 3 5 exonuclease activity. See also table 1. Taq polymerase is a thermostable dna polymerase i named after the thermophilic eubacterial microorganism thermus aquaticus from which it was originally isolated by chien et al.

Takara ex taq dna polymerase combines the proven performance of takara taq polymerase with the proofreading activity of an efficient 3 to 5 exonuclease for high sensitivity high efficiency pcr reactions it can also be used for long range pcr up to 20 kb from genomic dna templates and up to 30 kb from lambda dna templates. Product size price license quantity details. The final mg concentration in 1x standard taq reaction buffer is 1 5 mm. Mg and additives.

Taq dna polymerase is a high quality recombinant enzyme that is suitable for general and specialized pcr applications see figures tolerance of different primer t m values and specific amplification of long pcr products. The final mg concentration in 1x standard taq reaction buffer is 1 5 mm. Mg and additives. Its name is often abbreviated to taq or taq pol it is frequently used in the polymerase chain reaction pcr a method for greatly amplifying the quantity of short segments of dna.

Mg concentration of 1 5 2 0 mm is optimal for most pcr products generated with taq dna polymerase. The mixture is optimized for amplification of long dna templates routinely 20 kb and up to 48 kb depending on template type with high fidelity. Isolated from a recombinant source. Takara la taq is a mixture of taq polymerase with a proofreading polymerase.

Taq dna polymerase is a thermostable dna polymerase that possesses a 5 3 polymerase activity 1 2 3 and a 5 flap endonuclease activity 4 5. Qiagen pcr buffer. The magnesium free 10 buffer also includes a separate tube of 25 mm mgcl 2 for optimization. However mg can be further optimized in 0 5 or 1 0 mm increments using mgcl 2.

This supports satisfactory amplification of most amplicons. Taq dna polymerase was originally isolated from the thermophilic eubacterium thermus aquaticus bm a strain lacking taq i restriction endonuclease. It is supplied with 10x standard taq reaction buffer which is detergent free and designed to be compatible with existing assay systems. Optimal polymerization activity is achieved at 75 80 c with half maximal activity at 60 70 c lawyer et al 1993.

Innovative qiagen pcr buffer has been developed to save time and effort by reducing the need for pcr optimization. Mg concentration of 1 5 2 0 mm is optimal for most pcr products generated with taq dna polymerase. Ex taq polymerase has a higher fidelity than standard taq with a.