lipid peroxidation dna damage by malondialdehyde

This is a harmful process that can negatively affect several cellular structures such as membranes lipids proteins lipoproteins and deoxyribonucleic acid dna 16 21.

Lipid peroxidation dna damage by malondialdehyde. Lipid peroxidation is an autoxidation process initiated by the attack of free radicals e g. Contents of 8oxodg in dna can be quantified by hplc with an ec detector and by an immunochemical method elisa. As stated before if in excess free radicals and oxidants give rise to a phenomenon known as oxidative stress. Malondialdehyde results from lipid peroxidation of polyunsaturated fatty acids.

Lipid peroxidation leads to a chain reaction that generates fur ther mutagenic and genotoxic molecules i e malondialdehyde 4 hydroxynonenal and. It most often affects polyunsaturated fatty acids because they contain multiple double bonds in between which lie methylene. Dna rna damage lipid peroxidation and protein oxidation nitration. Oxidative stress can be measured indirectly by measuring the levels of dna rna damage lipid peroxidation and protein oxidation nitration rather than a direct measurement of reactive oxygen species.

First lipid peroxidation was studied for food scientists as a mechanism for the damage to alimentary oils and fats nevertheless other researchers considered that lipid peroxidation was the consequence of toxic metabolites e g. Oh o 2 and h 2 o 2 on phospholipids or pufa of the membranes of cellular or subcellular components resulting in the formation of various sorts of aldehydes ketones alkanes carboxylic acids and polymerization products these products are highly reactive with other cellular components and. Detrimental effects of free radicals on human health. Lipid peroxidation is the chain of reactions of oxidative degradation of lipids it is the process in which free radicals steal electrons from the lipids in cell membranes resulting in cell damage this process proceeds by a free radical chain reaction mechanism.

Lipid peroxidation products may cause damage to dna and formation of 8 oxo 2 deoxyguanosine 8oxodg is a marker of oxidative damage to dna. It is a prominent product in thromboxane a2 synthesis wherein cyclooxygenase 1 or cycloxygenase 2 metabolizes arachidonic acid to prostaglandin h2 by platelets and a wide array of other cell types and tissues. These oxidative stress markers are more enduring than reactive oxygen species. Lipid peroxidation can be described generally as a process under which oxidants such as free radicals attack lipids containing carbon carbon double bond s especially polyunsaturated fatty acids pufas.

Ros can play and in fact they do it several physiological roles i e cell signaling and they are normally generated as by products of oxygen metabolism.