la taq dna polymerase with gc buffers
Advantage gc genomic la dna polymerase. Q5 master mixes contain dntps mg and a proprietary broad use buffer requiring only the addition of primers and dna template for robust amplification regardless of. Advantage hf 2 pcr kit.
Add 0 5 to 2 5 units of dna polymerase per 50 μl reaction see manufacturers recommendations for example add 0 5 μl of sigma 0 5 units μl taq dna polymerase.
La taq dna polymerase with gc buffers. Gmp grade pcr selection guide. Is a latin abbreviation for quantum satis meaning the. Takara la taq is a mixture of taq polymerase with a proofreading polymerase. Sterile distilled water to obtain a 50 μl final volume per reaction as pre determined in the table of reagents q s.
Gmp grade products takara taq hot start. Titanium taq sp. Combines taq dna polymerase and a dna proofreading polymerase to enable amplification of very long dna templat read more primestar max dna polymerase choose for speed convenience and highest fidelity. What length of product can be made by q5 high fidelity dna polymerase.
To use the calculator select your dna polymerase type in or paste your primer sequences and provide your final primer concentration. Q5 dna polymerase is composed of a novel polymerase that is fused to the processivity enhancing sso7d dna binding domain improving speed fidelity and reliability of performance. Primestar hs dna polymerase. A separate method is used for t m and annealing temperature calculation of reactions with taq based dna polymerases.
The mixture is optimized for amplification of long dna templates routinely 20 kb and up to 48 kb depending on template type with high fidelity. Rr002a takara la taq dna polymerase mg2 free buffer 125 units. Primestar gxl polymerase is our most robust high fidelity polymerase available for challenging targets gc rich sequences excess template long amplicons it is a modified version of the primestar hs enzyme that includes an elongation factor to provide unsurpassed processivity. There is a precipitate in the bottom of the buffer tube.
La taq dna polymerase with gc buffers. Primestar gxl dna polymerase provides efficient pcr amplification even for the toughest scenarios including. 240 county road ipswich ma 01938 2723 978 927 5054 toll free 1 800 632 5227 fax. Primestar hs with gc buffer.
High fidelity pcr advantage hd dna polymerase. Academia edu is a platform for academics to share research papers. Product size price license quantity details. T m values annealing temperature and other data are automatically generated.
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