illumina tagment dna enzyme and buffer small kit manual

To strip off the tn5 add 5 μl of nt buffer nextera xt dna sample preparation kit to the 20 μl solution containing the tagmented dna from step 29. Fifty thousand nuclei sample were tagmented with tagment dna tde1 enzyme and buffer kit illumina 20034198 in 50 μl reaction volume of transposition buffer 25 μl 2 td buffer tagment dna. Pipet the solution up and down a few times.
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