dna pmol calculator
For all calculations let s assume we have 22 nmol of a dna primer containing 16 bases. From lyophilized powder primers that you purchase from. Choose a dna rna genome editing qpcr calculator from neb a leader in production and supply of reagents for the life science industry.
Fortunately there are many easy methods to help you do the maths you need in the lab.
Dna pmol calculator. Ultramer dna oligos are long single and double stranded synthetic dna sequences. The web bench is the essential companion to the biologist bringing informational resources and a collection of tools calculators to facilitate work at the bench and analysis of biological data. With their increased length and purity ultramer dna oligos have increased utility in a variety of molecular biology applications including but not limited to. Easily create a stock solution by allowing the resuspension calculator take the guesswork out of dissolving your oligo.
Proprietary dna synthesis equipment permits rapid high quality synthesis of nucleic acids. Delivered amount of dry oligo in tube micromoles μmol nanomoles nmol picomoles pmol. 1 pmol of pbr322 dna. Opools oligo pools are pooled high fidelity single stranded dna sequences that are compatible with a variety of applications.
Here we tell you about the different ways to calculate primer concentration depending on the starting material. Calculations can be the bane of laboratory work. 1 pmol of 1 000 bp dna 0 66 µg. Also calculate molarity of solutions perform molar conversions calculate dilutions and perform other calculations common in molecular biology labs.
Check out the full online bench here. 1 µg of 1 000 bp dna 1 52pmol 3 03pmol of ends 1 µg of pbr322 dna 0 36pmol dna. Dna calculations to convert µg to pmol for double stranded and single stranded dna convert micrograms of dna to pmol ends calculate vector insert molar ratio and convert od260 readings to µg ml. Dna molecular weight calculator dna molecular weight is an important parameter for molecular biology.
This platform is the same proprietary synthesis platform used to make idt ultramers.
nanoliter scale dna assembly utilizing the nebuilder hifi cloning kit with the echo 525 liquid handler beckman coulter












































































