dna oligo molecular weight calculator

Also displayed for your convenience are the gc content melting temp tm and the reverse complement of the oligo sequence you enter. Dna calculations to convert µg to pmol for double stranded and single stranded dna convert micrograms of dna to pmol ends calculate vector insert molar ratio and convert od260 readings to µg ml. Our handy oligo analysis tool calculates molecular weight mw extinction coefficient e260 pmoles µg pmoles od and µg od.
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The oligocalc dna mw calculations assume that there is not a 5 monophosphate anhydrous molecular weight a n x 313 21 t n x 304 2 c n x 289 18 g n x 329 21 61 96. Molecular weight also known as molecular mass is an important parameter of dna rna protein and formula. Moreover oligo analyzer 3 1 allows you to blast your primer with ncbi.

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Dna oligo molecular weight calculator. This is known collectively as the human genome. In humans the nucleus of each cell contains 3 10 9 base pairs of dna distributed over 23 pairs of chromosomes and each cell has two copies of the genetic material. Dna molecular weight typically for synthesized dna oligonucleotides. Data generated from in house esi ms instruments.

This tool calculates melting temperature tm molecular weight mw millimolar extinction coeff od μmol μg od for oligos. With this primer design tool provided by integrated dna technologies the user can analyze the input sequence up to 255 bases obtaining helpful data. Please right click in sequence tab to insert internal modification. The measured molecular weight of each oligo is within 0 01 of its expected molecular weight.

Also calculate molarity of solutions perform molar conversions calculate dilutions and perform other calculations common in molecular biology labs. These calculators are useful and user friendly research tools offering the relative professional and exact datas. Dna rna and protein synthesis. The genetic material is stored in the form of dna in most organisms.

Qc data shows high purity of 185 nt and 189 nt ultramer dna oligos. Gc content molecular weight complementary sequence a representative image of the hairpin self dimer and heterodimer structures formation of the primer sequences. The molecular weight of water is 18 02 g mol while it could be millions for many complex organic molecules. Synthetic dna or rna oligonucleotides obtained by solid phase synthesis are employed in a majority of molecular biology applications such as pcr real time pcr sequencing site directed mutagenesis single nucleotide polymorphism snp assays microarrays and various therapeutic applications.