dna gyrase vs topoisomerase

A review in 2001 suggests that cytotoxicity of fluoroquinolones is likely a 2 step process involving 1 conversion of the topoisomerase quinolone dna complex to an irreversible form and 2 generation of a double strand break by denaturation of the topoisomerase. Dna gyrase is found in all bacteria but absent from higher eukaryotes. These binding sites can be defined as points of biochemical reaction crucial to the survival of the bacterium.
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A range of different binding sites has been identified including ribosomes penicillin binding proteins dna topoisomerase gyrase and the cell membrane itself. Acts on the lagging strand to remove primers and replace them with dna 2. In contrast topo i opposes dna gyrase by relaxing the negatively.

Match the function of each dna polymerase in e.

Dna gyrase vs topoisomerase. Not involved in replication but used for dna repair. Across all forms of life dna gyrase is the only topoisomerase that can create negative supercoiling and it is because of this unique ability that bacterial genomes possess free negative supercoils. The bactericidal action of ciprofloxacin results from inhibition of the enzymes topoisomerase ii dna gyrase and topoisomerase iv both type ii topoisomerases which are required for bacterial dna replication transcription repair and recombination. The crucial binding site will vary with the antibiotic class.

Norfloxacin does not bind to dna gyrase but does bind to the substrate dna. The enzyme dna is the topoisomerase involved in dna replication.