500 pmol dna

A minimum of 24 and 96 oligos required for 96 and 384 well plates respectively.

500 pmol dna. These include metric prefixes spectrophotometiric conversions agarose polyacrylamide gel resolutions etc. Purified pcr products 1000. After thawing the solution can be stored at 4 for up to 2 months. The optimal mass dna mass pei ratio will need to be empirically determined for each new batch of 1 mg ml pei and for each cell line.

10 µl of 2x nebuilder hifi dna assembly master mix was incubated with 6 dna fragments 4 fragments of 1 000 bp and one fragment of 1 152 bp with 80 bp overlap and a vector of 3 373 bp 20 bp overlap 0 05 pmol each in a final volume of 20 µl at 50 c for 60. You can use these tables to aid your dna rna protein quantification electrophoresis gel preparation protein synthesis and other research activities. After 2 months discard the tube and thaw a new working stock. Neb recommends a total of 0 03 0 2 pmols of dna fragments when 1 or 2 fragments are being assembled into a vector and 0 2 0 5 pmols of dna fragments when 4 6 fragments are being.

500 pmol scale requires a minimum of 96 oligos. Final dna per well 100 ng 500 ng 2500 ng final lipofectamine 2000 reagent per well 0 2 0 5 μl 1 0 2 5 μl 5 0 12 5 μl co transfection of plasmid dna and sirna transfect plasmid dna and sirna at the same time using lipofectamine 2000 reagent by adding 30 pmol 0 6 μg of sirna per 1 μg of dna. 6 10 kb 80 ng µl 800 ng 10 kb 100 ng µl 1000 ng. Plasmids 6 kb 50 ng µl 500 ng.

Dna length include vector template concentration in 10 µl. Template volume per reaction. Protocols io also provides an interactive version of this protocol where you can discover and share optimizations with the research community. Shipped dry or resuspended to your specifications.

Genscript provides several useful tables of common biological constants and conversions. Dna kits are the tools of choice for automated sizing. Bp bp area total size bp in cv pg µl pmol l 100 2 000 395 8 51 254 12 4 283 33 1 713 8 ab. Complexing dna p3000 reagent lipofectamine 3000 reagent sirna lipofectamine 3000 reagent 96 well 0 2 100 μl 2 x 5 μl 0 1 µg 0 2 µl 0 3 µl 3 pmol 0 3 µl 48 well 0 5 250 μl 2 x 12 5 μl 0 25 µg 0 5 µl 0 75 µl 7 5 pmol 0 75 µl 24 well 1 500 μl 2 x 25 μl 0 5 µg 1 µl 1 5 µl 15 pmol 1 5 µl.

Your primer concentration µm pmol µl your primer volume per reaction.